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Factors that may affect transpiration rates are light, heat, and wind. However, exposure to extremes of heat or wind may cause the plant to close its stomates rather than transpire more.

Student Name __________________________ Date____________

LAB REPORT EVALUATION MATRIX (Biology 156)

GOOD AVERAGE POOR NOT PRESENT

(BUT NEEDED)

ABSTRACT to include the following (10 pts)

250 words or less _______ ________ _______ _______

problem investigated _______ ________ _______ _______

methods _______ ________ _______ _______

major results _______ ________ _______ _______

conclusions _______ ________ _______ _______

significance of findings _______ ________ _______ _______

TOTAL _______

INTRODUCTION to include the following (20 pts)

sufficient background info on organism/system

with citations _______ ________ _______ _______

problem clearly defined _______ ________ _______ _______

purpose and goals _______ ________ _______ _______

hypothesis/prediction _______ ________ _______ _______

TOTAL _______

MATERIALS AND METHODS to include the following (20 pts)

summary of process _______ ________ _______ _______

purpose of a procedure _______ ________ _______ _______

no excess mundane details _______ ________ _______ _______

TOTAL _______

RESULTS to include the following (15 pts)

Tables and graphs clearly labeled _______ ________ _______ _______

Narrative includes summary tables and graphs _______ ________ _______ _______

TOTAL _______

DISCUSSION to include the following (20 pts)

interpretation of results _______ ________ _______ _______

Explanation of significance of results _______ ________ _______ _______

has related discussion of results to the goals,

hypothesis and purpose of experiment, including

conclusions _______ ________ _______ _______

includes citations _______ ________ _______ _______

TOTAL _______

LITERATURE CITED to include the following (5 pts)

listed alphabetically _______ ________ _______ _______

cited properly within narrative of paper _______ ________ _______ _______

correct format of citation _______ ________ _______ _______

TOTAL _______

WRITTEN EFFECTIVELY to include the following (10 pts)

written clearly _______ ________ _______ _______

Title page _______ ________ _______ _______

use of metric system _______ ________ _______ _______

each paragraph conveys a single major idea and has

topic sentence at beginning _______ ________ _______ _______

pages numbered _______ ________ _______ _______

avoidance of I and me statements _______ ________ _______ _______

TOTAL _______

Lab Report Grade ________

Using Transpirometers Must be put in your own word

Preparing the Plant (this can be done hours before the experiment is to be conducted):

1. Select a plant that has a section of stem that is of slightly larger diameter than the tan

rubber tubing attached to the pipette tip.

2. Cut the plant off at its base, below where the diameter is slightly larger than the rubber

tubing. Immerse cute end in pan of water.

3. Immerse the pipette with rubber tubing attached in the pan of water to remove all air from

the pipette and tubing. This may require pinching and squeezing the tubing. All air

bubbles must be removed.

4. With the stem submerged, cut the plant again at the point where the stem is slightly larger

than the rubbing tubing. Keep the cut stem under water at all times.

5. Slide the rubber tubing over the cut stem.

6. Hold the plant and tubing out the water and check for leaks. If air percolates into the tip

of the pipette right away then there is a leak. If there is a leak, resubmerge and try sliding

the tubing onto the stem farther.

Preparing the Apparatus:

1. Flush the system by opening the screw valve below the reservoir. Flush until no bubbles

leave the micropipette then clamp off the micropipette using the spring clamp. Continue

flushing until no bubbles are present in the line where the plant will be inserted.

2. Insert the plant and pipette combination into the tube. Wrap a paper towel around the

stem of the plant and insert the plant into the open clamp. Clamp just enough to hold the

plant upright.

3. Open the spring clamp on the line leading to the micropipette.

4. Open the screw valve on the reservoir just enough for water to drip from the micropipette

and then close the screw valve. Blot the drop from the tip of the pipette.

5. If the leaves of the plant are very wet, gently blot them dry.

6. Transpiration should be noticeable within a few minutes as water is drawn from the

micropipette.

Measuring Transpiration Rates:

1. The micropipette is marked at the 100 μL point. Students can measure the length to the

mark (usually about 88 mm) and then calculate a conversion factor (88 mm/100 μL).

Using the conversion factor they can calculate the volume transpired per unit time.

2. Factors that may affect transpiration rates are light, heat, and wind. However, exposure

to extremes of heat or wind may cause the plant to close its stomates rather than transpire

more. This might be something to try at the end of a lab period.

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